A quadrant streak technique was used on a nutrient agar plate using the Alternative 4. Tables 1 and 2 lists the tests, purposes, reagents used and the results of each test. It helps to isolate a possible a gram + and a gram -. The prelab must be completed prior to the day of the experiment. These were identified as Berb 1, Berb 2 and Berb 3. Using a wire loop, a small sample of the numbered test tube was plated on a nutrient agar using the quadrant streak method 4. Healthy adults will make a full recovery from this infection within a week.
Once the two colonies were found, they were isolated on separate agars for growth. The gram negative bacteria was done through elimination process following the Unknown Chart given by the Lab Professor. In this experiment, researchers classified an unidentified bacterium based on its physical structure, colony morphology, optimal conditions and metabolic properties. Based off of my results. The next biochemical test that was performed was a H2S test. Observations of any change or growth were properly recoded afterwards.
Everything must be sterilized and precautions must be taken to stop bacteria from the air getting in. What is the environmental pathway for the vector of this disease? Bacteria, Enzyme, Growth medium 2145 Words 7 Pages Microbiology-2460 Lab-003 March 31,2008 Lab Report-Escherichia coli Abstract The purpose for this lab report was to identify and inform of an unknown bacteria that has been causing a patient to have lower abdominal and pelvic pain. Escherichia coli is quite a unique bacterium due to the fact that some strands can cause a lot of harm, disease, and sickness to animals and humans, while some strands are a part of a healthy flora inside the body 3. The sample collected was extracted from intestinal region. Sometimes, disease-associated strains produce toxins which promote serious infections in the body. Most microbiology lab reports do not have an Introduction and begin with the Purpose section.
Each test performed, provided us with some key information about the unknown microbes in question and how the bacteria function. Next day, a gram stain was done as explained on Number 4 above. A second isolation was completed and when observed it confirmed that only two bacterium grew. It is a common misconception that all bacteria are harmful, when in fact, we have billions, if not trillions living inside us Swartzburg, 2009. The unknown bacteria is Staphylococcus aureus. Creamy 2 — darker off white, creamy appearance growth. Experiments using bacterial cultures are quite dangerous because if any unwanted bacteria mixes in with the experiment or gets on our hands it can contaminate experiments.
First to be prepared was the stationary phase, or chromatographic paper, which in this case was filter paper. While identifying the bacteria, it is important… Unknown bacteria determined to be Alcaligenes faecalis because of its morphological, physiological and metabolic properties. Knowing what you are dealing with can allow one to knowing the causative agent of a disease and how it can be treated. Unknown A, Escherichia coli, was gram-stained to determine the bacterial shape. That test tube only had a gram negative bacterium in it. In order to tell whether or not an organism tests positive for citrate utilization, Bromthymol blue dye is added to the agar. The citrate test determines if a bacterium possess citrate-permease as citrate is the only source of carbon in the medium.
Determine the mass of the unknown solid to the nearest 0. The unknown was identified using differential tests to determine its metabolic and morphology characteristics. In order to complete this test, several steps were taken. Any observation of color change were recorded. Once the molecules are converted to pyruvate many products can be made all depending on the pH of the environment. The bacterium were incubated and grown at 37 degrees for four days. The binding of antigens to the.
The second test performed was a Urea Test which also gave a clear positive result confirming that unknown gram positive bacteria as Staphylococcus epidermidis because Staphylococcus aureus gives a negative result. This experiment will also determine if our bacteria is a fermenter of sugars and if it is catalase positive. After determining the Gram-negative reaction, specific tests were performed. Gram-positive cocci shaped bacteria were identified using the microscope. That is why I got false negative result.
Introduction My unknown organism 6 is Morganella morganii, which is a gram-negative bacillus rods commonly found in the environment and also in the intestinal tracts of humans, mammals, and reptiles as a normal flora. The medium used in this test is the Simmons Citrate Agar, which is a type of utilization media. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. The items that appeared to be water soluble were the baking soda, the unknown substance, cornstarch, and possibly the baking powder hard to tell because it mostly became bubbles that stayed and didn't go away. Recently though, the microorganism is becoming the common cause of nosocomial infections.